The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...
Abundance, richness, and diversity of fungi in Urtica dioica roots for the three primer pairs tested fITS7/ITS4, gITS7/ITS4, and 5.8S-Fun/ITS4-fun (Ta = 57 °C), the addition of PNA clamps (+PNA) and ...