Understanding melt curves for improved SYBR Green assay analysis and troubleshooting
idtdna.com
Understanding melt curves for improved SYBR Green assay analysis and troubleshooting
qPCR assays using intercalating dyes, such as SYBR Green dye, are an economical and effective tool for measuring gene expression. To interpret intercalating dye assays, users need to know how to analyze melt curves, and understand the benefits and limitations of such analysis. In this presentation, Nick Downey, PhD, covers melt curve basics and ...
Apr 3, 2015
Melting Curve Analysis PCR
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