Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

3D Animations - Experiments & Techniques: Polymerase Chain Reaction - CSHL DNA Learning Center

Daily CRISPR Updates on Instagram: "In a new study, researchers have presented a CRISPR-based DETECTR system for rapid detection of the destructive Chilli leaf curl virus (ChiLCV), targeting its AC1 gene. This method employs recombinase polymerase amplification (RPA) and achieves visualization through fluorescence or a lateral flow assay. The system detects ChiLCV at concentrations as low as 7 femtograms and distinguishes it from related viruses and pest damage. Validated with field samples, thi