How to perform a CRISPR Knockout Experiment
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How to perform a CRISPR Knockout Experiment
Due to CRISPR's unparalleled ease-of-use and affordability, gene knockout experiments are now more feasible than ever before! In this video, we will show you how we used the CRISPR Cas9 system to knockout the LIF gene in a colon carcinoma cell line. This is accomplished by the delivery of a Cas9 and sgRNA lentivector into the cell line followed ...
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